Journal: Genome Biology

Article Title: The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation

doi: 10.1186/s13059-020-02115-y

Figure Lengend Snippet: SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the Incucyte automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm

Article Snippet: OCR was normalized to confluence using an IncuCyte® ZOOM phase-only processing module (Essen Bioscience).

Techniques: MTS Assay, Boyden Chamber Assay, Staining, Standard Deviation, Expressing